How to keep surface treated frits from biofouling – FAQ

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ARTICLES, APPNOTES, FAQ (9184)
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September 11, 2021
Bill Ciccone

2.o™ HPLC Columns FAQ / Cogent Column Filters - FAQ / Column Hardware FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Metal Free HPLC Columns Information / Mobile Phase FAQ / Reversed Phase Columns FAQ / TYPE-C in General FAQ

Use non surfactant, Brij-35 to reduce biofouling

When many proteins get trapped on or adhere to stainless steel frits that are hydrophobic like our metal free surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins.

Brig-35 will not diminish or change the effectiveness of the metal free surface. It is important to work in low concentrations of any ionic polymer to minimize biofouling due to surfaces.

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