Ifosfamide Assay Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9187)
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August 17, 2021
Tanya

AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

A Reliable Method for an Antineoplastic Chemotherapeutic Drug

This Ifosfamide Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.8%. This Method shows reliability for analysis of this Pyrimidine analog that is an antineoplastic anti-metabolite.

Peak:

Ifosfamide

Method Conditions:
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-30P-3.9
Dimensions: 3.9mm x 300mm
Mobile Phase: (70:30) Water / Acetonitrile
Injection vol.: 25μL
Flow rate: 1.0mL / minute
Detection: UV @ 195nm
Sample Preparation: Ifosfamide 0.6mg / mL in DI Water

Note: Ifosfamide is an Alkylating and immunosuppressive agent used in Chemotherapy for the treatment of Cancers, including Testicular Cancer, Ovarian Cancer, Cervical Cancer, Osteocarcinoma, Bladder Cancer, and non-Hodgkin’s Lymphoma.

This Method was developed by and is presented courtesy of ARL- Eutech Scientific Services, Inc.

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