A Reliable Assay for Detection of a Chemotherapeutic  

This simple Isocratic Method may be used to quantitate this important drug.  The Method maintains consistent and reliable results as the Peak values were measured for week-long intervals.  The %RSD for Retention Times for both standards and sample were less than 0.7%.


Method Conditions:
Column: Cogent RP C18™, 5µm, 100Å
Catalog No.: 68518-30P-3.9 
Dimensions: 3.9 x 300mm
Mobile Phase: 70:30 DI Water / Acetonitrile
Injection vol.: 25μL
Flow rate: 1.0mL / minute
Detection: UV @ 195nm
Sample Preparation: Ifosfamide 0.6mg / mL in DI Water

Note: Ifosfamide, ( sold under the brand name Ifex®) is a chemotherapy medication used to treat a number of types of Cancer. This includes Testicular Cancer, soft tissue Sarcoma, Osteosarcoma, Bladder Cancer, Small Cell Lung Cancer, Cervical Cancer, and Ovarian Cancer. 

This Method was developed by and is presented courtesy of Eutech Scientific Services