A Reliable Assay for Detection of a Chemotherapeutic  

This simple Isocratic Method may be used to quantitate this important drug.  The Method maintains consistent and reliable results as the Peak values were measured for week-long intervals.  The %RSD for Retention Times for both standards and sample were less than 0.3%.

 

Peak:
Gemcitabine

Method Conditions:
Column: Cogent RP C8™, 5µm, 100Å
Catalog No.: 68508-25P
Dimensions: 4.6 x 250mm
Mobile Phase: 13.8g of Monobasic Sodium Phosphate and 2.5ml of Phosphoric Acid in 1L of DI Water
Injection vol.: 20μL
Flow rate: 1.2mL / minute
Detection: UV @ 275nm
Sample Preparation: Gemcitabine, 0.1mg / mL in DI Water

Note: Gemcitabine, with brand names including Gemzar, is an IV Chemotherapy medication and treats cancers including testicular cancer, breast cancer, ovarian cancer, non-small cell lung cancer, pancreatic cancer, and bladder cancer. 

This Method was developed by and is presented courtesy of Eutech Scientific Services