Epigallocatechin Gallate Analyzed with HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9187)
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September 6, 2021
Tanya

AppNotes Food and Beverages / AppNotes Library / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

A Reliable Method for a EGCG Analysis

This Epigallocatechin Gallate (EGCG) Assay produces Run to Run consistency and Precision with Retention Time RSD values of 0.02%. This is a sensitive HPLC-UV Method that may be used for quantification of EGCG in food and beverage formulations.

Peak:

Epigallocatechin Gallate

Method Conditions:
Column: Cogent Zx Similars C18, 5um, 100Å
Catalog No.: 65518-15P
Dimensions: 4.6mm x 150mm
Mobile Phase: 25:75 Acetonitrile / DI Water with 1.0% Acetic Acid
Injection vol.: 100μL
Flow rate: 1.0mL / minute
Detection: UV @ 280nm
Column Temperature: Ambient
Sample Preparation: Epigallocatechin Gallate 100μg / mL in DI Water

Note 1: Epigallocatechin Gallate is the ester of Epigallocatechin and Gallic Acid, and is a type of Catechin. EGCG is the most abundant Catechin in tea and is a polyphenol under basic research for its potential to affect human health and disease.

Note 2: The Cogent Zx Similars C18™ performs very similarly to Agilent’s Zorbax™ brand C18 HPLC Columns or similar C18 Columns. This makes it an ideal Alternate in USP Methods that use the L1 Classification and Agilent’s Zorbax™ brand C18 HPLC Columns.

This Method was developed by and is presented courtesy of ARL- Eutech Scientific Services.

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