Direct Adaptive HPLC Column Connectors Benefit – Tips

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February 28, 2022
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ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

How to Reduce Peak Broadening from Poor Column Connections

The spring-loaded “Direct Adaptive (Di-Ad™) HPLC Column Connectors” were designed for speed and convenience when changing between different column brands on your instrument. In the example below, we demonstrate how this error-free process using DiAd fittings can also reduce peak broadening from common poor connections.

Trace	Fitting Used	Height (mAU)	Plates (N)
Black	PEEK	31.33	6306
Red	Poorly Inserted PEEK	30.36	6023
Blue	Di-Ad™	35.37	7160

Method Conditions:
Direct Adaptive HPLC Column Connector: Double end fitting
Catalog No.: 49910-10-DD
Dimensions: 0.010″ ID x 1/16th” OD, 100mm long
Column: Cogent Bidentate C8™, 4μm, 100Å.
Catalog No.: 40008-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 40% Acetonitrile / 60% DI Water 0.1 % Formic Acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV 254nm
Sample Preparation: 0.1mg / mL Phenol in Mobile Phase.

Notes: Column Efficiency: Theoretical Plates (N) 1/2 Height N = 5.54((t_R/W)²)
t_R = Retention Time of Peak
W = Width of Peak Measured at 1/2 Height

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