How to Reduce Peak Broadening from Poor Column Connections
The spring-loaded “Direct Adaptive (Di-Ad™) HPLC Column Connectors” were designed for speed and convenience when changing between different column brands on your instrument. In the example below, we demonstrate how this error-free process using DiAd fittings can also reduce peak broadening from common poor connections.
|Trace||Fitting Used||Height (mAU)||Plates (N)|
|Red||Poorly Inserted PEEK||30.36||6023|
Direct Adaptive HPLC Column Connector: Double end fitting
Catalog No.: 49910-10-DD
Dimensions: 0.010″ ID x 1/16th” OD, 100mm long
Column: Cogent Bidentate C8™, 4μm, 100Å.
Catalog No.: 40008-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 40% Acetonitrile / 60% DI Water 0.1 % Formic Acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV 254nm
Sample Preparation: 0.1mg / mL Phenol in Mobile Phase.
Notes: Column Efficiency: Theoretical Plates (N) 1/2 Height N = 5.54((tR/W)2)
tR = Retention Time of Peak
W = Width of Peak Measured at 1/2 Height