A Simple ELSD Assay for Detection of a Bile Acid – aka Cholanoic  Acid 

This isocratic Method is used for routine analyses to quantify Deoxycholic Acid that has been used in manufacturing processes. Sample size is typically limited and sensitivity is crucial.  This Method maintains consistent and reliable results as shown in the 5 injection overlay below.  The UDC Cholesterol Column was chosen due to the shape of the molecule, as it is very similar to steroids.   This Column is good for selectivity of analyte shape and hydrophobicity.

 


Peak:
Deoxycholic Acid

Method Conditions:
Column: Cogent UDC Cholesterol™, 4µm, 100Å
Catalog No.: 69069-15P
Dimensions: 4.6 x 150mm
Mobile Phase: 80:20 Methanol / DI Water
Injection vol.: 1μL
Flow rate: 0.5mL / minute
Detection: ELSD, Temperature: 55°C, Gain: 9
Sample Preparation: Deoxycholic Acid  analytical standard 1mg / mL in DI Water

Note: Deoxycholic Acid in an injectable form is used to improve the appearance and profile of moderate to severe submental fat.  Deoxycholic Acid, also known as Cholanoic Acid and sometimes sold as Kybella™ or Belkyra™, is one of the secondary bile Acids, which are metabolic byproducts of intestinal bacteria and can be used to determine intestinal fat adsorption. Also used in research, when a micelle is formed with it, it can used as a mild detergent for the isolation of membrane proteins.