Why is Borosilicate Glass Reactive and Adsorptive? – FAQ

It is widely known that Borosilicate Glass is often covered with dissociable silanol groups, (about 6 groups / Square Nano-Meter) giving it a negative charge when in contact with solutions, especially water based diluents. Positively charged compounds (bases) will be adsorbed to these negative sites. Adsorption can be due to these silanols but there are […]

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What is Protein Oxidation? – FAQ

It is reported in the literature that Proteins can degrade when they react with Oxygen. This can occur when Proteins are stored in plastic containers and Oxygen permeates the walls of the Vials. Also, during gamma irradiation sterilization, free radicals can be generated in plastic containers that can cause degradation of the Proteins. RSA™ and […]

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Will RSA-Pro X Vials & Inserts Work for All Proteins? – FAQ

Not likely, but it will work for the vast majority of hydrophobic proteins. The RSA-Pro X™ Vials and Inserts have been used with many types of proteins, that are hydrophobic in nature, successfully and satisfactorily. However, there are so many different proteins, that making a blanket statement that they “will work” with All Proteins is […]

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Are RSA-Pro X Vials & Inserts Certified? – FAQ

Yes, a Certificate of Conformance for QC and Product Performance is available. The RSA-Pro X™ Vials and Inserts under go extensive QC testing before they are released for sale to the community. A “certificate” is available by contacting our Customer Service Department. Click Here for the Contact Information.

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Are RSA-Pro X Vials & Inserts the Same as Silanized Vials & Inserts? – FAQ

No, they are very different! Silanized vials coat glass using gaseous silanes under pressure. While Silanizing does convert hydrophilic glass into glass with a hydrophobic coating, RSA-Pro X™ Glass uses a very different surface treatment. The biggest difference as far as you are concerned is that Silanized Glass Vials are susceptible to degradation in the […]

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White Paper: Helpful HPLC Columns You May Not Know About.

This document is a two page “White Paper” that provides a interesting Dialogue between Professor Joseph Pesek and LCGC Magazine. The short discussion is about HPLC Columns that you may not know about but have been very useful to many labs around the world for many years. Attachment Helpful HPLC Columns You May Not be […]

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Acetaminophen Impurities Method with HPLC – AppNote

Acetaminophen – Alternate USP Method Acetaminophen and two of its major impurities were analyzed using a simple Mobile Phase. The Peak Shapes are very good and the Repeatability was good (%RSD = 0.01). Peaks: 1. 4-Aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes Method Conditions: Column: Cogent Bidentate C18™, 4µm, 100Å Catalog […]

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Comparison of Extracts in Jaboticaba Fruit Powder with LCMS – AppNote

Phenolic Compounds Separated with HPLC with LCMS This simple Analytical Method was used to determine the content of very potent compounds present in Jaboticaba extracts and quantified some its Phenolic contents in fermented/non-fermented fruit; estimated by using standard addition. It is important to know the quantitation of compounds before and after fermentation to optimize the […]

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Fosetyl-Aluminum Analysis with HPLC. Optimizing Tips – AppNote

You can use the Cogent Bidentate C18™ column in reversed phase (RP) with a high water content method. According to a 2014 third-party research article, if the analysis is done by reversed phase an ion pair agent is recommended to increase retention and reduce peak tailing. Here, 8 mM sodium sulfate was used in a […]

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PFAS Analysis using Glass or Plastic Vials – Tips and Suggestions

In order to take the most accurate accurate analysis of PFAS Special Care must be taken for storage and sampling. Glass and also low-density polyethylene (LDPE) materials are not recommended for storing PFAS samples. PFAS can adsorb to glass, particularly when the chemicals are stored in a glass container for extended periods of time. Recommended […]

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