Are RSA-Pro X Vials Recommended for PFAS Analysis – FAQ

RSA-Pro X™ Vials & Inserts are not recommended for PFAS or Per- and Polyfluoroalkyl Substances analysis with LCMS / MS or GCMS. During the conversion from RSA™ to RSA-Pro X™, the vials and inserts are exposed to PFAS. While some of these compounds of interest may not be detectable in HPLC, they may be detected, […]

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How to Keep Dursan Coated Frits from Biofouling – FAQ

Non surfactant, Brij-35 to Reduce Biofouling When many proteins get trapped on or adhere to Stainless Steel Frits that are hydrophobic like Dursan surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins. Brig-35 will not diminish […]

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White Paper: Helpful HPLC Columns You May Not Know About.

This document is a two page “White Paper” that provides a interesting Dialogue between Professor Joseph Pesek and LCGC Magazine. The short discussion is about HPLC Columns that you may not know about but have been very useful to many labs around the world for many years. Attachment Helpful HPLC Columns You May Not be […]

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Acetaminophen Impurities Method with HPLC – AppNote

Acetaminophen – Alternate USP Method Acetaminophen and two of its major impurities were analyzed using a simple Mobile Phase. The Peak Shapes are very good and the Repeatability was good (%RSD = 0.01). Peaks: 1. 4-Aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes Method Conditions: Column: Cogent Bidentate C18™, 4µm, 100Å Catalog […]

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Comparison of Extracts in Jaboticaba Fruit Powder with LCMS – AppNote

Phenolic Compounds Separated with HPLC with LCMS This simple Analytical Method was used to determine the content of very potent compounds present in Jaboticaba extracts and quantified some its Phenolic contents in fermented/non-fermented fruit; estimated by using standard addition. It is important to know the quantitation of compounds before and after fermentation to optimize the […]

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Fosetyl-Aluminum Analysis with HPLC. Optimizing Tips – AppNote

You can use the Cogent Bidentate C18™ column in reversed phase (RP) with a high water content method. According to a 2014 third-party research article, if the analysis is done by reversed phase an ion pair agent is recommended to increase retention and reduce peak tailing. Here, 8 mM sodium sulfate was used in a […]

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PFAS Analysis using Glass or Plastic Vials – Tips and Suggestions

In order to take the most accurate accurate analysis of PFAS Special Care must be taken for storage and sampling. Glass and also low-density polyethylene (LDPE) materials are not recommended for storing PFAS samples. PFAS can adsorb to glass, particularly when the chemicals are stored in a glass container for extended periods of time. Recommended […]

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EDTA Analysis with HPLC – AppNote

EDTA does not have a significant Chromophore, so to achieve UV Detection, in the Method shown below we used a pre-Column reaction of a Solution of Ferric Chloride with the Sample. The resulting EDTA/Fe3+ has significant UV Absorbance making this a very Sensitive Method. Ethylenediaminetetraacetic acid is extremely difficult to analyze by itself however in […]

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Dursan Coated HPLC Columns Comparison to PEEK Column Performance – Tips

Dursan is Excellent for any Compound that will Interact with Stainless Steel PEEK is a highly inert material which has been used in HPLC applications for years. However PEEK has some limitations: Mechanical strength: PEEK begins to flex at 3000 to 5000 PSI, making it unsuitable for UHPLC and other applications where high pressure is […]

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I heard that there is Not a Good Alternative to HILIC to Achieve Sufficient Retention for some compounds. Is this true? – FAQ

This is an untrue statement that can lead chromatographers to frustration as THERE IS an alternative mode of chromatography that surpasses HILIC in reproducibility, precision and the ability to separate both hydrophobic and hydrophilic compounds. It is well documented that Aqueous Normal Phase HPLC (ANP) is a very viable alternative to HILIC for polar compounds and in many cases superior […]

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