Argatroban analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9184)
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October 19, 2022
Tanya

AppNotes Cogent Phenyl Hexyl / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods

A simple method for this di-peptide

A rapid, sensitive, and reproducible method has been developed for this blood thinner. The data below, (an overlay of 10 chromatograms) illustrates how the compound can be adequately retained and detected using this straightforward method.

A phenyl ring in the column stationary phase provides strategic use of π-π Interaction with the analyte making possible the use of a very simple, Mass Spec-friendly mobile phase with formic acid as an additive.

Peak:
Argatroban

Method Conditions
Column: Cogent Phenyl Hexyl™, 4μm, 100Å
Catalog No.: 68539-15P
Dimensions: 4.6mm x 150mm
Mobile Phase:
—A: DI water
—B: Acetonitrile

Time (minutes)	%B
0	25
1	25
3	50
4	50
5	25
8	25

Injection vol.: 2μL
Flow rate: 1.0mL / minute
Detection: UV @ 215nm
Sample Preparation: 0.5 mg / mL in 50:50 acetonitrile / DI water
%RSD: <1.0%
t₀: 1.5 minutes
K’: 1.35

Notes: Argatroban is a synthetic direct thrombin inhibitor used for the prevention and treatment of thrombosis related to heparin use. It acts as a preventative of the formation of blood clots, which reduces the risk for stroke or other medical conditions.

Note 2: Capacity is determined using the following equation: k = (t_R-t₀)/t_O

t_R = Retention time of an analyte peak
t_O = Retention time of non-retained peak

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