Cyclobenzaprine Hydrochloride USP Assay – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9184)
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May 27, 2020
Tanya

AppNotes Alternate Generic Methods / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

This method demonstrates the USP Cyclobenzaprine Hydrochloride Extended-Release Capsules Assay.

As shown in the Chromatograms, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18 5μm column. This demonstrates a great alternative column for easy plug and play and this UPS Assay Method.

Method Conditions:
Column: Cogent RP C18™, 5μm, 100Å
Catalog No.: 68518-15P
Dimensions: 4.6 x 150 mm
Buffer: Add 3.7 mL of Methanesulfonic Acid per 1 L of Water and adjust with Diethylamine to a pH of 3.6
Mobile Phase: Acetonitrile, Methanol, and Buffer (25.5:21.0:53.5)
Injection vol.: 20μL
Flow rate: 1.5 mL/minute
Detection: UV @ 290 nm
Diluent: Acetonitrile and Water (50:50)
Standard solution: 0.12 mg/mL of USP Cyclobenzaprine Hydrochloride RS in Diluent

Most recently appeared in Pharmacopeial Forum: Volume No. 40(4) Page information: USP42-NF37 – 1177, USP41-NF36 – 1119, USP40-NF35 – 3602

Note: Cyclobenzaprine is a muscle relaxant and works by blocking nerve impulses that signal pain sensations sent to your brain. Cyclobenzaprine is used together with rest and physical therapy to relieve muscle spasms caused by painful conditions such as an injury.

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