Using Two Columns and two Modes of HPLC Together

If you connect the Cogent Bidentate C18™ & Cogent Diamond Hydride™ columns in parallel, but use the same mobile phase program on both columns, it will be difficult to have both Reversed Phase and Aqueous Normal Phase (ANP) modes operating simultaneously, therefore you will not get optimal results. A gradient starting at high organic may work well for the Cogent Diamond Hydride™ column but only very hydrophobic compounds will retain on the Cogent Bidentate C18™ column.

However, the attached article below describes a more sophisticated system where retention of both hydrophobic and hydrophilic analytes can be retained using both columns. The set-up and procedure are described here in detail.

Metabolomics studies involve not only known and predicted metabolites but also of a wide variety of ones that had not previously been predicted to exist. Due to the significant complexity of any metabolome, both the Cogent Bidentate C18™ and Cogent Diamond Hydride™ together give a much more complete picture than when used individually. You need appropriate instrumentation to get the best results. Both Columns are available in a discounted package: Metabolomics Kit Product Page


University of Melbourne Poster   Download File

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