We recommend a starting temperature of 25 °C for most applications. Generally you will see higher retention at higher temperatures and lower retention at lower ones with Aqueous Normal Phase (ANP) chromatography. This can sometimes be a useful tool for fine-tuning the retention/selectivity of the peaks.

Although temperature is a variable to consider during method development, we would suggest factors such as mobile phase organic content, pH, ionic strength, and so on have a greater effect on the separation. You could also use a higher temperature to lower the column pressure as well. This might be important with the 2.o™ column, which generates higher pressure than our standard 4µm phases when used under the same conditions.

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