The pH range of common buffers for HPLC. – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ
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July 6, 2013
jimmy

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions / Mobile Phase FAQ

The following table shows a list of common buffers and their working buffer ranges:

Buffer	pH Range
trifluoroacetic acid (TFA)	1.5-2.5
phosphoric acid/monobasic phosphate (pKa 1)	1.1-3.1
formic acid/formate	2.8-4.8
acetic acid/acetate	3.8-5.8
mono/dibasic phosphate (pKa 2)	6.2-8.2
ammonia	8.2-10.2
1-methylpiperidine	9.1-11.1
triethylamine	10.0-12.0

As for concentration, about 5mM is suitable for Cogent TYPE-C Silica™ columns while 25mM can be used with columns based on type B silica.

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