The following table shows a list of common buffers and their working buffer ranges:

Buffer pH Range
 trifluoroacetic acid (TFA)  1.5-2.5
 phosphoric acid/monobasic phosphate (pKa 1)  1.1-3.1
 formic acid/formate  2.8-4.8
 acetic acid/acetate  3.8-5.8
 mono/dibasic phosphate (pKa 2)  6.2-8.2
 ammonia  8.2-10.2
 1-methylpiperidine  9.1-11.1
 triethylamine  10.0-12.0

As for concentration, about 5mM is suitable for Cogent TYPE-C Silica™ columns while 25mM can be used with columns based on type B silica.

image_pdfDownload this Page