Glucosamine can be retained using an ANP gradient method with the Cogent Diamond Hydride™ HPLC column. Using either 4um particle size or 2.o™ (2.2um) columns did not affect retention times but efficiency was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:

The method conditions used were as follows:
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H2O + 0.1% formic acid
B: Acetonitrile +  0.1% formic acid

time (min)    %B
0                   80
5                   30
7                   30
8                   80
post time 4 min

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