How do retention and efficiency of glucosamine compare using 4um vs. 2.o™ Cogent Diamond Hydride™ HPLC columns?

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AppNotes Cogent RP C8
ARTICLES, APPNOTES, FAQ
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May 28, 2013
jimmy

ARTICLES, APPNOTES, FAQ / Diamond Hydride FAQ

Glucosamine can be retained using an ANP gradient method with the Cogent Diamond Hydride™ HPLC column. Using either 4um particle size or 2.o™ (2.2um) columns did not affect retention times but efficiency was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:

The method conditions used were as follows:
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H₂O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid

time (min)    %B
0                   80
5                   30
7                  30
8                  80
post time 4 min

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