It is reported that HILIC columns can be less robust than traditional reversed-phase (RP) columns. Partly this is due to the fact that the surface can be easily contaminated and is hard to clean. In other cases, the bonded group is not very robust and is susceptible to being cleaved from the surface in mobile phases used for HILIC retention.

Columns used for aqueous normal phase (ANP) are more robust if used properly and can be readily cleaned using a variety of techniques.

Also HILIC columns are known to require large equilibration times due to formation of the adsorbed water layer on the surface. This can affect robustness if the column is not fully equilibrated before starting the injections. Here Cogent TYPE-C Silica™ columns have another advantage since this water layer is not involved.