Why polar compounds are generally more strongly retained in Aqueous Normal Phase HPLC (ANP) when ionized

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ARTICLES, APPNOTES, FAQ
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March 25, 2013
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ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

The highest polarity is generally found in ionized compounds due to the fixed charge on the compound. Therefore in aqueous normal phase (ANP) maximum retention is attained with the highest polarity, i.e. when the compounds are ionized.

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