Why polar compounds are generally more strongly retained in Aqueous Normal Phase HPLC (ANP) when ionized

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AppNotes Cogent RP C8
ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
ONLINE TOOLS
SALES POLICIES

March 25, 2013
jimmy

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

The highest polarity is generally found in ionized compounds due to the fixed charge on the compound. Therefore in aqueous normal phase (ANP) maximum retention is attained with the highest polarity, i.e. when the compounds are ionized.

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