If you connect these two columns in parallel but use the same mobile phase program on both columns, it will be difficult to have both reversed phase and aqueous normal phase mechanisms operating simultaneously and therefore not get optimal results; a gradient starting at high organic may work well for the Cogent Diamond Hydride™ column but only very hydrophobic compounds will retain on the Cogent Bidentate C18™ column.
However, the attached article below describes a more sophisticated system where retention of both hydrophobic and hydrophilic analytes can be retained with both columns. The set-up and procedure are described here in detail.
You need appropriate instrumentation to get the best results. Metabolomics studies involve not only known and predicted metabolites but also of a wide variety of ones that had not previously been predicted to exist. Due to the significant complexity of any metabolome, both the Cogent Bidentate C18™ and Cogent Diamond Hydride™ together give a much more complete picture than when used individually.
Univ Melbourne 2D poster.pdf 1.1 Mb Download File