I am looking to quantify and qualify milk samples with HPLC. In terms of quantification I am would like a column that will resolve the common caseins i.e. alpha, beta and kappa as well as the common whey proteins alpha lactalbumin, beta lactoglobulins and bovine serum albumin (BSA). We need a rapid and robust column as we are also looking to establish profiles of various milk samples. This is more qualitative at this time but separation is still important as we look to build up a finger print type profile.
I’ve read a number of applications from the Cogent website and have been impressed by the columns provided.
Casein is a phosphoprotein and the Cogent Bidentate C8 300 HPLC columns could have some interesting advantages and benefits for you because these analytes may be successfully separated in the Aqueous Normal Phase (ANP) mode. Putting in the other proteins complicates things but our experience with peptides indicates that this strategy might work for proteins too. Being proteins, these should all be UV detectable and mostly all proteins can be detected at low wavelength, 210-215 nm. Perhaps the combination of the wide pore, C8 ligand and hydride surface might provide some unique capabilities for this type of analysis. I am just speculating because we have not done much work with proteinsat this time.
For more information about the Cogent Bidentate C8 300 wide pore HPLC columns, call or click here: Cogent Bidentate C8 300