To start work in normal phase mode, a simple switching procedure is recommended.
A simple 30 minute procedure allows switching from one mode to another.

Procedure:

A – Moving from Reversed Phase to Normal Phase HPLC; pump 100% Methanol for 15 minutes at 1 mL/min. Flow Rate, followed by 15 minutes 100% Methylene Chloride. The Column is ready to be Equilibrated with Mobile Phase for NP-HPLC.

B – Moving from Normal Phase to Reversed Phase HPLC; pump 100% Methylene Chloride for 15 minutes at 1 mL/min. flow rate, followed by 15 minutes 100% Methanol. The Column is ready to be equilibrated with Mobile Phase for RP-HPLC.