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ARTICLES, APPNOTES, FAQ (9187)
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What is the elution order for analytes in the Resolution Test Mixture supplied in the HSQ kit – Tech Information

KB Home/ARTICLES, APPNOTES, FAQ /ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / ARTICLES, APPNOTES, FAQ / Qualification Information / HPLC Qualification / ARTICLES, APPNOTES, FAQ / HPLC Suggestions and Information / ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / Qualification FAQ / ARTICLES, APPNOTES, FAQ / Qualification Information / What is the elution order for analytes in the Resolution Test Mixture supplied in the HSQ kit – Tech Information

July 7, 2012
Bill Ciccone

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ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Qualification / HPLC Suggestions and Information / Qualification FAQ / Qualification Information

The peaks / analytes elute in the following order for the PQ Kit™:

Uracil
Theophylline
Caffeine
8-Chloro-Theophylline

For more recent and more efficient HPLC’s, the HSQ Kit™ has a different Resolution Test Mixture with one additional, earlier eluting peak. This additional peak allows the HSQ software to best determine your system’s extra column volume. This extra peak is not required but may be preferred.

Uracil
Theobromine
Theophylline
Caffeine
8-Chloro-Theophylline

Click HERE for PQ and HSQ kit ordering information and pictures.

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