LC-MS method for Anthocyanin- Fruit or Vegetable Extracts

In this analysis, the presence of Peonidin-3-O–Glucoside was confirmed in two out of four extracts. The peaks were symmetrical and retained beyond the dead volume.

Due to the MS detection and comparison of the retention time, it was possible to find the peak for Peonidin-3-O-Glucoside among many peaks with similar m/z values present in the A1 extract. The method after validation can be used for quality control of commercial fruit extracts as well in studies of bioactivities of this important class of compounds.


Peak:
Peonidin 3-O-glucoside 463 m/z [M+]

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

   Time (minutes) %B
0 15
4 80
6 80
7 15

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.4 mL/minute
Detection: ESI – POS – Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Four proprietary fruit or vegetable extracts were analyzed.  Samples were marked A1 to A4.
t0: 0.4 minutes

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