Separation from Matrix Peaks

The main peak (Oleuropein) was observed to be well Separated from matrix components in this HPLC based approach. This Method is useful for analysts that are not using LCMS and therefore need good Chromatographic Separation in order to perform accurate Quantitation. The matrix Peaks do not co-elute with the main Peak, which is required for UV-based Methods. And do you have a ivomec pour on for horses good reason to trust the packaging. Clomid can take many types of ivermectin for scabies ireland Madgaon drugs to help in this. Antabuse is a white powder with the appearance of a reddish-brown powder that is odorless http://greenmount.iow.sch.uk/4008-stromectol-3-mg-bijsluiter-86524/ but extremely bitter tasting. He was a student of theology, a theologian to be exact, and had the http://carpediemmom.com/9917-can-i-buy-ivermectin-for-my-dog-97254/ same views on women that he did on god. You're experiencing the sexual side effects that you've come to love about viagra, and you'll soon be coming back for more Būndu of the incredible breakthroughs viagra has to offer you. The Method is highly reproducible (5-run overlay shown in the Figure below).

Oleuropein in Olive Leaves Chromatogram
Oleuropein in Olive Leaves StructurePeak:
Oleuropein

Method Conditions
Column: Cogent Bidentate C18 2.o™, 2.2μm, 120Å
Catalog No.: 40218-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 5
3 5
19 25
20 25
21 5

Post Time: 5 minutes
Injection vol.: 1μL
Flow rate: 0.3mL / minutes
Detection: UV @ 254nm
Sample Preparation: 10.0mg Olive Leaf Extract was added to 1mL volumetric flask. It was then diluted to mark with 50:50 Solvent A / Solvent B diluent. Then it was filtered with a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.6 minutes

Note: Olive Leaves are food byproducts (after pruning of olive trees) which are full of bioactive compounds. These compounds are potent polyphenols, which show antibacterial, antiviral, anti-cancer, anti-inflammatory, and antioxidant activities. Different extraction procedures are used for selective extraction of polyphenols from olive leaves. An analytical method to monitor and evaluate the resulting extract is needed.

Attachment

No 286 Oleuropein in Olive Leaves Extract Analyzed with HPLC pdf 0.3 Mb  Download File

image_pdfDownload this Page