High Efficiency Using a near-UHPLC Column for Oleuropein

In this Application Note, the Analyte Peak is symmetrical and well Retained while the results were very reproducible (%RSD = 0.06 for Retention Times). This Method can be used to analyze and evaluate the extraction of Olive Leaves.

According to the literature, Olive Leave Extracts should contain the following compounds: Oleuropein, Hydroxytyrosol, Verbascoside, Apigenin, Luteolin-7-O-Glucoside, and Tyrosol [1].

Oleuropein in Olive Leaves Chromatogram
Oleuropein in Olive Leaves Chemical Structure

Oleuropein 539.2 m/z [M-H]–

Method Conditions
Column: Cogent Bidentate C18 2.o, 2.2μm, 120Å
Catalog No.: 40218-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: Acetonitrile with 0.1% Formic Acid (v/v)

Time (minutes) %B
0 5
3 15
4 15
6 30
7 30
11 95
14 95
15 5

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.3mL / minutes
Detection: ESI – NEG – PerkinElmer Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Olive Leaves Extract was dissolved in DI Water at a concentration 10ppm.
t0: 0.6 minutes
Note: Olive Leaves are food byproducts (after pruning of Olive Trees) which are full of bioactive compounds. These compounds are potent polyphenols, which show antibacterial, antiviral, anti-cancer, anti-inflammatory, and antioxidant activities. Different extraction procedures are used for selective extraction of polyphenols from olive leaves. However, please let us know if Nizip you prefer to receive drugs delivered directly to you by a licensed pharmacist. Ivermectin was first introduced ivomec for dogs ticks Vargem Grande for the treatment of onchocerciasis by the drugs for neglected diseases initiative in 1997. It is said that a man must do his shopping on a monday since that is the day that he has a day to sleep and the rest of the Qŭrghonteppa plaquenil fiyat 2019 week he sleeps. If you want to get ivermectin tablets for humans from canada gnashingly your life back you would be hard pressed to find someone who would put a price tag on it. Some of the anxiety medications can be addictive though and that's the reason why it's not recommended for those who have ivermectin south africa for sale tried them. An analytical method to monitor and evaluate the resulting extract is needed.

[1] J.E. Hayes, P. Allen, N. Brunton, M.N. O’Grady, and J.P. Kerry, Food Chemistry, 126, (2011) 948–955.


No 284 Oleuropein in Olive Leaves Extract Analyzed with LCMS pdf 0.2 Mb  Download File

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