Analysis of Imitrex® API from Tablet Formulations

This AppNote Method for Analysis of a challenging compound (Sumatriptan) has several advantages over other Methods. This Analysis can be performed using a higher concentration of organic solvent, which is much more suitable for electrospray MS Detection.

This AppNote provides robust and repeatable (%RSD 0.1 and lower) results.

Sumatriptan (Imitrex) Tablets Chromatogram


Peak:
Sumatriptan 296.1427 m/z [M+NH3]+

Method Conditions
Column: Cogent Diamond Hydride 2.o™, 2.2μm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 90
4 30
6 30
7 90

Post time: 3 minutes
Injection vol.: 1µL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: Six 25mg strength tablets of Imitrex® were crushed and a portion containing 40mg of the API was weighed out. The powder was suspended in an Solvent A / Solvent B (1:1) Mixture, vortexed, and filtered through a 0.45μm Syringe Filter (MicroSolv Tech Corp.). The patients were discharged from the hospital the next Barra do Piraí day when no adverse reaction or treatment failure was observed. For this reason, there are usually safer alternatives Brzesko to using weed and cialis. Nolvadex is an oral, nonselective, reversible inhibitor of cyp2c9 and cyp3a and is approved https://shop.schiffinger-schuster.at/15506-ivermectin-for-dogs-south-africa-1631/ for the treatment of osteoporosis in canada. Ivermectin has a long history how fast does oral ivermectin kill scabies as a key ingredient in treating head lice [3]. It could also prevent some ivermectina cpr roaring heart diseases, diabetes and kidney diseases. A Sample for injection was diluted to final concentration of 0.0005 μg / mL.
t0: 0.9 minutes

Note: Migraine is a common disorder, with symptoms of unilateral headache accompanied by nausea and / or vomiting. Sumatriptan is an anti-migraine drug sold under the trade name Imitrex®. It belongs to a class of drugs called Selective Serotonin Receptor Agonists.

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