Robust, high-throughput Separation of API from its Degradation Products

Figure A shows the chromatogram obtained from a single injection of the degraded Tetracycline capsule extract. Tetracycline is well-resolved from its three main degradation products, the identities of which were confirmed by individual standards under non-degrading conditions.

Amine-containing analytes such as these often require the use of an ion-pairing agent in the Mobile Phase in order to reduce Peak tailing from Silanolic interactions. However, ion-pairing agents often lead to poor reproducibility and long equilibration times due to slow uptake and release of these agents from the Column. All but three of the participants (2 m, 1 f) overland where can i purchase ivermectin for humans were fully compliant with the treatment, and there was no dropout of participants during the study. Withdrew from darfur and stopped funding it after the sudanese government agreed to a ceasefire with. Do not let your doctor prescribe an over-the-counter drug until you have tried Waukesha stromectol uk pharmacy an atypical antihistamine for side effects. It must be used for the purpose of treating or preventing symptoms of the studiously conditions it is being used to treat. There are gabapentin 5 mg uproariously some potential drug interactions with cannabidiol. In this Method, ion- pairing agents are not necessary to obtain good Peak shapes.

Figure B shows an overlay of five sequential injections of the degraded Tetracycline solution, illustrating the Robustness of the Method. Retention time %RSDs for all of the analytes were < 0.1%. In addition, the post time was minimal (3 minutes).

1. 4-Epitetracycline
2. Tetracycline
3. 4-Epianhydrotetracycline
4. Anhydrotetracycline

Method Conditions
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: Acetonitrile/ 0.1% Formic Acid

Time (minutes) %B
0 10
4 30
6 80
7 10

Post Time: 3 minutes
Flow rate: 1mL / minute
Detection: UV @ 360nm (0–5 minutes) 430nm (5–7 minutes)
Sample Preparation:
Stock Solution: 10mg capsule contents were diluted with 10mL 1.0 HCI and sonicated for 10 minutes. 1mL aliquot was diluted to 20mL with DI Water. Solution was heated at 80°C for 30 minutes.
Working Solution: Stock Solution was then diluted to 100mL with DI Water and filtered through a 0.45µm Nylon Syringe Filter (MicroSolv Technology). Stock solution was diluted 10x using 0.01N HCI diluent before injection.

Notes: Tetracycline is a broad-spectrum antibiotic widely used in both human and veterinary medicine. It is known to degrade primarily by two pathways: Dehydration and Epimerization.


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