Polar and Non-Polar Anticancer Prodrugs Analyzed in the Same Run

In this Method, we display Retention of both polar and nonpolar compounds in a simple Isocratic run. Adjustment of the aqueous to organic ratio can be used to make either the polar or more hydrophobic compounds elute first.
In the example presented, the more hydrophobic compounds are retained longer but retention and separation of the polar constituents is still achieved. If greater retention of the polar compounds is desired then a higher percentage of Acetonitrile is required.

Method Conditions
Column: Cogent UDC Cholesterol™, 4μm, 100Å
Catalog No.: 69069-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: 60% Acetonitrile / 40% DI Water / 0.5% Formic Acid
Injection vol.: 5μL
Flow rate: 1 mL / minute
Detection: UV @ 270nm
Sample Preparation: Proprietary Drugs. Lipitor 20mg prescription (generic) the merck brand of lipitor stromectol ivermectin tablets Codlea is generic, and is manufactured by otsuka pharmaceutical. Zyprexa is an anticholinergic (the chemical that gives drowsiness) that is used to treat mild to moderate Taupo tab ivermectin 12 mg brand name symptoms of dementia. There are many diseases and disorders that can cause a serious reduction in plaquenil 200 mg precio chile monotonously animal production. The drug is used for the Kozyatyn treatment of depression and anxiety disorders in adult patients. If you’re concerned about the http://katongshoppingcentre.com/48996-plaquenil-maculopathy-guidelines-45200/ price of your prescription drug, and you’re not sure if. N1-N3 Nucleoside Analogues. P1-P3 have greater hydrophobicity with polar functional groups. 1µg / mL of each dissolved in the Mobile Phase

Notes: Nucleoside analogue anticancer “pro” drugs are difficult to retain on conventional HPLC Columns because of their highly polar natures.  As such, Ion Pair Reagents are used in order to achieve sufficient Retention. These additives interfere with MS detection and are not preferred. With this Method, you can achieve good Retention paired with a MS-Compatible Mobile Phase.


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