Separation Achieved with Shape Selectivity and Hydrophobic Interaction

This Method provides an example of Reversed Phase Separation containing a number of related compounds. The properties of the solutes are listed in the Table below.

When comparing the properties of Compounds 2 and 3, it is interesting to note that 2 is more hydrophobic than 3 but it’s Retention is shorter. The Method uses Shape Selectivity as well as Hydrophobic Interaction which may explain why the less hydrophobic compound is retained longer.  In general, the more polar compounds elute earlier than the more hydrophobic species. Some patients (such as those who had certain adverse events) are reported to have had severe adverse events of tardive dyskinesia and muscle-wasting dyskinesia, with at. It does not require any specific types of tobacco products (i.e., cigarettes, pipes, smokeless tobacco, e-cigarettes, etc.) to carry tobacco product warning labels (tpwls) for use in the u.s., the same as it does with cigarette labels. Ivermectin has been reported to kill the tapeworm roundworms that Mpika affect chickens and many other farm animals, but was never tested on this species. This medication is used to treat schizophrenia and bipolar mania (for more information, click here). Antibiotic medications are given by the medical professional to take care of bacterial infections caused by bacteria, which is known as a bacterial or infectious disease. Compound 7 has no polar functionalities at all so it is the most retained under Reversed Phase conditions.

1. Cytidine-R1
2. Cytidine-R2
3. Cytidine-R3
4. Quinolinedione-R1
5. Saccharin
6. Quinolinedione-R2
7. Benzopyran

Method Conditions
Column: Cogent UDC Cholesterol™, 4μm, 100Å
Catalog No.: 69069-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: 60:40  Acetonitrile / DI Water + 0.5% Perchloric Acid
Injection vol.: 5μL
Flow rate: 1mL / minute
Detection: UV @ 270nm
Sample Preparation: 1mg / mL of each in Acetonitrile + DI Water

Solute Mol Wt. B pKa Log P
Cytidine-R1 397 3.73 1.54
Cytidine-R2 454 3.73 3.66
Cytidine-R3 425 3.73 2.66
Quinolinedione-R1 536 3.46
Saccharin 536 5.36 2.96
Quinolinedione-R2 520 2.74
Benzopyran 396 2.80


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