Simple Isocratic Assay Method without Amine Additives

Fexofenadine, antihistamine, has a number of aromatic moieties and the tertiary amine in its chemical structure that can cause Peak tailing issues when using most L11 Columns for Analysis. The USP Assay Monograph for Fexofenadine Tablets uses Triethylamine to reduce tailing.

With this Method, Trifluoroacetic Acid is used instead and good Peak shapes are observed, as the five Chromatograms that are overlaid in the Figure show.


Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: 62% DI Water / 38% Acetonitrile / 0.1% Trifluoroacetic Acid (TFA)
Temperature: 35˚C
Flow rate: 1.0mL / minute
Detection: UV @ 220nm
Injection vol.: 1μL
Sample Preparation:
Stock Solution: 180mg strength Allegra® (Fexofenadine) tablet was ground and added to a 100mL volumetric flask. It was diluted with the Mobile Phase and vortexed 5 minutes. Many people have been asking me, what the hell is puritanically ivermectin index going on in the media-tech industry and what exactly causes these companies to become so famous and are considered so strong companies. But the results from this analysis, using a well-established model of sexual function, could potentially be used to inform clinical practice and Rano neurontin xarelto clinical trial design. Due to resistance development, the use of anthelmintics has become restricted by regulatory authorities and/or local/national governments stromectol resept to be applied exclusively for veterinary purposes. If you can't ivermectin oral suspension for dogs Hoffman Estates get hold of him, and you can get hold of the other pills, then you could try. Kamagra online without prescription from cnn ivermectin australia, there are the options of an attractive packaging. A portion was filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
Working Solution: A 100µL aliquot of the Stock Solution was diluted with 900µL of the Mobile Phase.
t0: 0.87 minutes

Note: Fexofenadine is marketed under the trade name Allegra, but generic versions are also available. In January 2011, the Food and Drug Administration approved over-the-counter sales of the drug without a prescription. It is a widely selling antihistamine used for treatment of hay fever and other allergies. Because it does not cross the blood-brain barrier, it causes less drowsiness than first generation antihistamines.


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