Analysis using a Simple Mobile Phase.
In this Method two important amino acids: L-(+)–alpha-phenylglycine and L-phenylalanine, were Separated.
C18 Columns used today which may be present in every Analytical Laboratory may not be able to Retain underivatized Amino Acids. They usually elute at or near the “void volume” with other polar compounds.
Peaks:
1. L-(+)-Alpha-Phenylglycine
2. L-Phenylalanine
Method Conditions
Column: Cogent Silica-C™, 4μm, 100Å
Catalog No.: 40000-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Formic Acid
—B: Acetonitrile / 0.1% Formic Acid
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Injection vol.: 2μL
Sample Preparation: 0.3mg / mL of each sample dissolved in 50:50 Acetonitrile / DI Water / 0.5% Formic Acid.
t0: 0.85 minutes
Notes: Alpha – amino acids are precursors for many important chemical entities (for example isoindolines), which are essential to the discovery of new drugs.
Attachment
No 48 Phenylglycine & Phenylalanine Analyzed with HPLC pdf 0.2 Mb Download File
