Excellent Peak Shape with an Easy to Run Method

This Method is easy to prepare, use and reproduce. Note the Excellent Peak Shape for this Compound. This Methodology will give a long Column life and many Reproducible Runs.


Peak:
Warfarin

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 52% Acetonitrile / 48% DI Water / 10mM Sodium Phosphate Monobasic
Temperature: 40°C
Injection vol.: 10μL
Flow rate: 1.5mL / minute
Detection: UV @ 280nm

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It is important to note that using Phosphate can alter HPLC Columns and once you use Phosphates with this Column, you should dedicate to Phosphate based Methods.

Attachment

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