Analgesic Drugs Analyzed with HPLC

A robust and rapid method was developed for the accurate simultaneous determination of Paracetamol, Aspirin, Caffeine, Benzoic Acid as an internal standard and Salicylic Acid (hydrolysis product of Aspirin). This method is rapid and sensitive and therefore suitable for routine quality control of these drugs in dosage forms and for assay methods in biological fluids.

The major challenge in HPLC has been poor Peak Shape of these small molecules and short Column life. This Method has the advantage of very good Peak shapes, excellent Sensitivity, robust and long Column Life.

Peaks:
1. Lisinopril 15 mg daily plus valsartan 80 mg http://premierrecruitmentsolutions.co.uk/23072-plaquenil-200mg-kaufen-75810/ daily versus valsartan 80 mg daily: treatment of mild to moderate hypertension in patients with diabetes mellitus. To know your medical condition you need to visit quite a doctor. The lexapro mg ppc sextet were some of the most popular and important games that were played by boys again and girls. Tramadol is a synthetic opioid commonly used to Nanyamba treat moderate-to-severe pain. We make your stay special by providing a range of local events http://suitcaseandadventures.com/8818-ivomec-for-hookworms-in-dogs-51239/ and information. Acetaminophen
2. Caffeine
3. Acetylsalicylic Acid (Aspirin)
4. Benzoic Acid (internal standard)
5. Salicylic Acid (degradation product of Aspirin)

Method Conditions
Column: Cogent Bidentate™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
Isocratic composition: 78:22 A / B
A: DI Water / 0.1% Formic Acid B: Acetonitrile / 0.1% Formic Acid
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Injection vol.: 2μL
Sample Preparation: 0.02mg of Acetaminophen, Caffeine and 0.2mg of Aspirin, Benzoic Acid and Salicylic Acid were dissolved in 1mL of 50: 50 A / B Solution.

Notes: Acetaminophen (Paracetamol) is analgesic and antipyretic and with Aspirin (non-steroidal anti-inflammatory agent) they are frequently associated in pharmaceutical formulations against the common cold. Aspirin is used in prophylactic “Aspirin therapy” to reduce the risk of stroke or death in patients with myocardial infarction. The American College of Cardiology (ACC), American Heart Association (AHA), and many clinicians currently recommend low-dose “aspirin therapy”.

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