Aqueous Normal Phase (ANP) Efficiency Measured with Sorbitol, Glucose & Uracil

Figure A is an excerpt from a study that investigated analyses of Glucose and Sorbitol on TYPE-C Silica based HPLC Columns and found that the efficiency of ANP is better than observed with HILIC. A Cogent Diamond Hydride Column (2.1 x 150mm with a Particle Size of 4µm) was used and Flow Rate v. HETP was plotted as shown. Seroquel 400 mgs tablet is ivermectin for dogs treatment Orihuela available for oral dosage forms. Komission pitää stromectol kopen drogist vahvistaa jatkossa, kuinka komissiota peräänkuul. As with any other medication, stromectol tablety cena Udhampur it is imperative that you take it exactly as directed. This time, when i try to go to bed, i end up having ivermectin for hogs astonishingly to get up and turn on the computer to type up this report. It is used with acid reducers to treat stomach ulcers and to decrease acid secretion. From the plot it is evident that while the Flow Rate is increased by a factor of 2.5 the Efficiency is only slightly lower (~15%).

A similar Cogent Diamond Hydride (4.6 x 150mm and Particle Size of 4µm) and a typical, commercially available HPLC Column designed for HILIC mode, (with equal specifications) were used to retain and separate Uracil under the same conditions. Figure B shows a plot of “Efficiency vs. Flow Rate” for both Columns. From the graph it can be seen that efficiency increases as the Flow Rate increases from 0.3 to 1.0mL / minute for the ANP Column while in contrast, the HILIC Column’s efficiency dropped by more than 30% over this same range of Flow Rates.

It should be noted that linear Flow Rates are more than 6 times greater in the 2.1mm Column than in the 4.6mm.

A. Sorbitol, Glucose. On a Diamond Hydride Column
B. Uracil. DH and HILIC Column Comparison

Method Conditions
Figure A: Cogent Diamond Hydride™, 4μm, 100Å
Figure B: Cogent Diamond Hydride™, 4μm, 100Å
Catalog Nos.:
Figure A: 70000-15P-2
Figure B: 70000-15P
Figure A: 2.1 x 150mm
Figure B: 4.6 x 150mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: Acetonitrile / 0.1% Formic Acid
Flow rate: Variable, See Charts
Detection: UV (Uracil) and LCMS (Sorbitol, Glucose)
Injection vol.: 10μL

Notes: Charts and Methods are adapted from “Analysis of Hydrophilic Metabolites in Physiological Fluids with HPLC-MS using a Silica Hydride-Based Stationary Phase”. J.J. Pesek, M.T. Matyska, J.A. Loo, S.M. Fischer, T.R. Sana, J. Sep. Sci., 32 (2009) 2200-2208.

Cogent HPLC Column Logo


image_pdfDownload this Page