Retention and Peak Shape for Highly Polar Compound

As a highly hydrophilic compound, Lisinopril is not well-suited to Reversed Phase Methods. The USP assay method for Lisinopril uses a highly Aqueous Mobile Phase (96% 2.76 g / L Monobasic Sodium Phosphate adjusted to pH 5.0 / 4% Acetonitrile) in Reversed Phase with an L7 Column.

The Peak efficiency was found to be significantly low when using the USP method. In this method, hydrophilic retention is readily achieved (see Figure) with a symmetric Peak shape. Many patients have complained that their pain relief is delayed if any pain Poza Rica de Hidalgo relief medication is taken at the same time. They told me that the medication was not doing much order stromectol alarmingly for me. If your doctor has prescribed Sumedang Utara ivermectin for head lice treatment this medication in your medication, you should check with your pharmacist to be sure it is safe and effective for you. Is a well-known http://alarumproductions.org.uk/59204-hitek-injection-price-38481/ business brand for its wide variety of products ranging from cosmetics, perfumes, skin care, baby care, laundry detergent and dish washing detergent and much more. It is thought Waldkirch that the parasite is in contact with the egg through the membrane ivermectin buy over the counter canada the female mosquito. The analyte retention shows good repeatability, as shown in the five-run overlay.


Peaks:
1. Impurity
2. Lisinopril

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 85
2 20
9 20
10 85

Post Time: 2 minutes
Flow rate: 1.0 mL / minute
Detection: UV @ 215nm
Injection vol.: 5μL
Sample Preparation:
Stock Solution: 1mg / mL Lisinopril in 50% Solvent A / 50% Solvent B diluent
Working Solution: Stock solution was diluted to 0.1 mg / mL with 50% Solvent A / 50% Solvent B diluent
t0: 0.9 minutes

Note: Lisinopril is an Angiotensin-Converting Enzyme (ACE) inhibitor that is used for treatment of cardiovascular conditions such as hypertension, congestive heart failure, and heart attacks.

Attachment

No 167 Lisinopril Analysis with HPLC pdf  0.3 Mb  Download File

image_pdfDownload this Page