Properly cut a fused silica capillary for CE – How To

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ARTICLES, APPNOTES, FAQ (9187)
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April 11, 2012
Bill Ciccone

KB Home

Capillary Electrophoresis Information / CE Accessories Information / CE and CZE FAQ / Controlled Flow Capillary FAQ / How To... Information / Zero Flow Capillary FAQ

A Recommended Cleaving or Cutting Procedure for Simplus Brand or any Bare Fused Silica Capillaries is as follows:

Cleaving Procedure:

Place the Capillary over a large Diameter Radius Surface such as large pipe or tube.
Keep the Capillary under slight Tension.
Hold a fresh, Clean Cleaving Stone at an approximately 30º angle to the Capillary.
Drag the edge of the Cleaving Stone across the Fiber penetrating the Polyimide Coating. Do not apply excessive Pressure.
Pull the capillary HORIZONTALLY until it breaks (see illustration below).
If the Capillary will not break, the Polyimide has not been properly cut.
Repeat the above Steps.

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