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16 How to Condition a CEC Column

It is very important that you condition the capillary properly before using it. DO NOT CONDITION YOUR COLUMN LIKE A CE COLUMN: IT WILL BE DESTROYED. Instructions for Conditioning and Installation of Milli-phase…

17 How to Condition a CelerityCE™ Column

View information about CelerityCE capillaries It is very important that you condition the capillary properly before using it. DO NOT CONDITION YOUR COLUMN LIKE A CE COLUMN: IT WILL BE DESTROYED. Instructions…

18 How to Condition CE100-SA Controlled & Zero EOF Capillaries For CE

Capillary Conditioning Procedure: Never Use Strong Bases on these Capillaries Carefully remove the capillary from the shipping/storage container. Remove protective polyimide coating by using 98% fuming…

19 How to condition Cogent Type-B columns for storage

Long Term Storage: Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to use non-buffered mobile phase. Then purge the column with 8 column volumes…

20 How to condition Cogent TYPE-C columns for storage

Storage Solvents for Reverse Phase (RP) or Aqueous Normal Phase (ANP): If RP and ANP are your desired next use for these columns, storage in a mixture of 40% Distilled Water that is acidified with 0.1%…

21 How to Condition Cogent TYPE-C Silica™ HPLC Columns for Storage

This article describes how to prepare the Cogent TYPE-C Silica™ columns for initial use when you first receive them as well as how to store them for either overnight or long-term to prevent column degradation.…

22 How to fix a Cogent HPLC column that has had ammonium acetate precipitated in it

Question: I was using a method with 16 mM ammonium acetate in the mobile phase. I stored the column in pure acetonitrile immediately afterwards, but now I observe peak splitting when using the ammonium…

23 How to integrate a main peak when a shoulder peak is present

It is always better to have baseline separation between a main peak and any nearby minor peaks. However, this is not always possible and you may have to deal with integration of a peak with a minor shoulder…

24 How to optimize methods using Cogent TYPE-C HPLC columns

In the aqueous normal phase mode (ANP) the organic modifier is usually 60% or higher (up to and including 100%) of the mobile phase. Typically to work in ANP, you should also start with a mobile phase…

25 How to perform an Ohm's Law plot for optimal CE results

Ohm’s Law Plot to Optimize your Separation MicroSolv CE Primer Select the optimal voltage setting for your separation Maximize the Efficiency of your method Learn the upper Voltage Limits of your method…

26 How to prepare a 0.05% solution from a pharmaceutical tablet for HPLC injection

Preparation of a 0.05% solution for HPLC injections depends on the pharmaceutical tablet strength (dosage) and how much volume of diluent you use. In most cases we would recommend using your intended…

27 How to properly cut a simplus™ bare fused silica capillary

Cleaving Procedure: The recommended cleaving or cutting procedure is as follows: Place the capillary over a large diameter radius surface. Keep under slight tension. Hold a fresh, clean cleaving stone…

28 How to Use Bubble Point to Filter Water through Hydrophobic Membranes...

Bubble point pressure is the differential pressure required for a liquid to flow through a dry filter membrane. The differential pressure is either a positive pressure on the liquid layer on top of the…

29 I am seeing a loss in concentration of heparin with HPLC when using syringe filters. What do you recommend?

Heparin has strongly acidic sulfonate groups (negatively charged) that will bind with nylon filter membrane material. PTFE is not suitable because of its hydrophobicity. The most appropriate membrane…

30 Instantly search a document for exact text matches (Ctrl-F)

On a PC, you can instantly find any specific text that is found in a document using Ctrl-F (Command-F on a Mac). This will work for webpages, Word files, pdfs, and any other document with text (note:…

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