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1 Best sparging stone pore size to use for carbonating applications

A 10um pore size gives the best air bubbles for carbonation applications, such as carbonated beverages. A variety of product options are available for 10um sparging stones, such as different diameters…

2 Can I make the PTFE syringe filter more hydrophilic?

Yes, if you are wiling to introduce Methanol into your method. For a 25mm device, if you use 15-20mls of methanol as a blank filtrate, the membrane will give you less back pressure after that for aqueous…

3 Can you use a sparging stone for removal of dissolved oxygen?

Yes, Sparging stones have been shown to remove dissolved oxygen (or CO2) from water or wine. The efficiency of sparging is influenced by many factors, such as bubble size, contact time between the gas…

4 Filtering proteins and which membrane to use

Filters should not remove proteins or any other dissolved compounds from a solution. Filters have one purpose and that is to remove un-dissolved particles no matter what they are. Some filters, depending…

5 How should I handle the sparging stones/inlet filters to avoid contaminating my mobile phase?

The sparging stones/inlet filters should not be touched by human hands. Always wear clean standard laboratory gloves of use forceps when you handle them. Do not use powdered gloves, as these can introduce…

6 How To Condition 47mm Nylon Membranes for Use with the Mobile Phase Filter Degasser...

Filter membranes with smaller pores sizes such as 0.45µm have higher bubble points (the differential pressure required to draw air through a wet membrane). The bubble point is a function of the pore…

7 How to Use Bubble Point to Filter Water through Hydrophobic Membranes...

Bubble point pressure is the differential pressure required for a liquid to flow through a dry filter membrane. The differential pressure is either a positive pressure on the liquid layer on top of the…

8 Is stainless steel or titanium better for inlet filters?

Stainless steel is suitable for most applications but titanium is recommended for analyses involving biological matrices. Stainless Steel Inlet Filter Product Page © Copyright MicroSolv Technology Corporation,…

9 Protecting the analytical column: When to use guard columns vs. inline filters

All filters including an inline filter are designed solely for preventing un-dissolved particulate matter from reaching the analytical column. Note that the analytical column does have porous frits on…

10 Sometimes I see extra peaks in my samples and sometimes I am not using the same filter. Do you have any ideas?

Yes, the “proper” way to use any syringe filter is to dispose of the first few milliliters that comes from the filter. If there are any extractables or if there is going to be any adsorption to the…

11 Spurious Peaks from nylon syringe filters with a DI water solvent with Electrochemical Detection.

When using electrochemical detection, spurious peaks in your chromatogram may be observed if a nylon syringe filter is used with a DI water solvent. The anionic peaks are most likely nitrate extractable…

12 What are the differences between Nylon and PTFE filters for my filtration application?

There is a bit of a misconception in the field regarding PTFE v. Nylon membranes for filtration using syringe filter devices and which membrane can be used with different solutions and or sovlents. PTFE…

13 What is a “steady state” for filters?

A filter could have charged sites on it or a small amount of extractables. Most manufacturers, including MicroSolv, produce filters with the lowest extractables possible. If these extractables are soluble…

14 What is an extractable that comes from syringe filters?

An extractable from a syringe filter or any device for that matter, is any chemical that is present in your sample after filtering that was not there before and the source is the filter. Most of the time,…

15 What is the difference between AQ™ brand and low cost syringe filter brands?

AQ™ syringe filters are made to a higher standard of quality such as extractables, adsorption, flow design and have a 3 piece leak proof plastic device. AQ™ filters are supplied with CofA for HPLC…

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