MicroSolv Technologies Knowledge Base

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Mobile Phase Handling

1 Are nylon filters compatible with chloroform?

All nylon filters are not recommended for use with halogenated solvents such as chloroform or with strong acids (1N and stronger). For these liquids, you should consider using PTFE filters. View filtration…

2 Are the MicroSolv sparging stones good for "ozonating" water.

Yes, you can use any of our stainless steel sparging stones with ozone to "ozonate" water. It is recommended to use 10um pore size stones. Click here to learn more about our sparging stones © Copyright…

3 Are the sparging stones made with a nitric acid rinse?

The sparging stones are manufacturered without using nitric acid. Sparging Stone Product Page © Copyright MicroSolv Technology Corporation, all rights reserved.

4 Are your plastic coated glass bottles autoclavable?

No, they are not. If the bottles were to be autoclaved, it could cause some discoloration because of the plastic coating. In addition, autoclaving by heat will melt the plastic, and autoclaving by gas…

5 Can I use benzene as a mobile phase solvent?

Benzene can be used as an HPLC solvent in theory. It is compatible with standard HPLC instrumentation and will not damage an HPLC column. However, we would not recommend using this solvent for two main…

6 Can I use SS tubing with my MicroSolv sparging stone?

Our sparging/filter stones are designed to work with low pressure tubing such as PTFE, FEP, ETFE, or PFA. We offer two types of sparging devices. One type is will require the tubing to be inserted and…

7 Can the non safety-coated mobile phase reservoir bottles be used on a hot plate?

The non safety-coated borosilicate glass mobile phase reservoir bottles can be heated on a hot plate under certain conditions. If you are boiling water (100 °C) on a hot plate using this product for…

8 Compatibility guidelines for Safety-Coated Mobile Phase Reservoir Bottles

The plastic coating of the Safety-Coated Mobile Phase Reservoir Bottle is not compatible with dry heat in excess of 110 °C, an open flame or other direct heat source, use on hot plates, autoclaving above…

9 Do the MicroSolv mobile phase bottle caps have holes to insert tubing through?

Yes, there are three holes in the top of the cap which are threaded for use with standard low pressure male nuts as shown below in the image. This cap has 2 different size through holes which allows for…

10 How can I obtain a flat baseline in gradient mode besides using "blank subtraction"?

There is another way you can obtain a flat baseline besides "blank subtraction" but it can be a little tricky. It is called "absorbance matching". Typically when your B solvent is mostly acetonitrile,…

11 How much pressure can your mobile phase handling reservoirs handle?

These mobile phase reservoir bottles are not pressure rated and are not intended to be pressurized. Normally the bottle stands above the HPLC-system and this provides enough pressure for the solvent to…

12 How much volume can the 47mm membrane filters handle?

The 47mm membrane filters are used for vacuum filtration of solvents. In most cases a filter can be used as long as it is not blocked with particulate matter. In most applications you will use the filters…

13 How often do you need to change filters for the Filter/Degasser?

The frequency at which the filter membrane has to be replaced is dependent on the sample's volume of undissolved particles. The more particles, the more frequently it has to be replaced and vice versa.…

14 How should I handle the sparging stones/inlet filters to avoid contaminating my mobile phase?

The sparging stones/inlet filters should not be touched by human hands. Always wear clean standard laboratory gloves of use forceps when you handle them. Do not use powdered gloves, as these can introduce…

15 Should I use methanol as the organic solvent for ANP?

Methanol is generally not recommended for most ANP applications however a few scientists have seen success with it. Instead, it is best to use polar aprotic solvents such as acetonitrile. If using ELSD…

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