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CE Capillary Electrophoresis

1 Adsorption equilibria in CE using CElixir-pH.

The answer is very simple, the adsorption equilibria is shifted from your analyte to The Accelerator Solutions. The reasons for this are many but the main reason is the number of charges on your sample…

2 Buffer Additives for HPCE

Additive Function Organic Solvents Modify EOF Urea Solubilize Proteins Denature oligonucleotides Surfactants MECC, Separation of neutral Species Charge reversal of Capillary Wall Cellulose Derivatives…

3 Buffer Hints

Making Your Own Buffers As a general rule in making your own buffers, they are most effective within one to two pH units of its pKa. It is best to begin developing your HPCE method with a low concentration…

4 Can I use the CElixir Plus Kit to analyze Fe (II) or Fe (III)?

Iron and other metals will form a complex with aminopyridine (which is a component of the CElixir Plus Kit), so you will not be able to detect it. For this reason you need or to use another buffer. ©…

5 Changing Capillary Dimensions

When you change the ID of your capillary in your CZE system, the following changes can be expected. It is important to be aware of these effects. Joule Heating: An increase ID or length of your capillary…

6 Changing Conductivity in CZE

When you change the Conductivity of your CZE method, the following changes can be expected. Joule Heating: Reducing or lowering the conductivity will reduce the amount of Joule Heating in your capillary.…

7 Changing pH can Affect These Parameters in CZE

When you change the pH of your CZE system, either the buffer or the conditions inside of your capillary, the following changes can be expected. Electro osmotic Flow: 1.Lower pH extremes suppresses EOF.…

8 Choosing a Proper Buffer in CZE of Proteins

The diverse types and characteristics of proteins makes selecting a proper buffer very challenging. Factors to consider when choosing a buffer for proteins are: A. The protein’s pI and pK’s B. The…

9 Correction for late migrating peaks

The normalization of electropherograms is required as slower moving peaks generate a wider peak width than the faster moving peaks. The area of any peak is a function of both its peak width and height.…

10 Effects of Changing EOF in CZE

When you change the Electro Osmotic Flow (EOF) of your CZE method, the following changes can be expected. Joule Heating: Reducing or lowering the EOF will reduce the amount of Joule Heating in your capillary.…

11 Hint on Serum Proteins and HPCE

When performing Serum Protein or iso LDH-isoAmylase-iso CK or iso phosphotase analyses, it may happen that a protein (immunoglobulin or enzyme) binds to another protein (such as an immuno complex) or…

12 How can I analyze iron (FeII) and iron (FeIII) by CE?

There are two viable options for analysis of iron (II) and iron (III) by CE. Option 1. Add EDTA and analyze the iron-EDTA complexes. Use the CElixir OA Kit because the complexes will be anionic. Option…

13 How do I use the CE Window Maker for a different size detection windows?

The MicroSolvCE Window Maker™ comes supplied with a heating module suitable for burning a detection window that is approximately 2mm wide. Replacement Modules for 1, 4, or 7mm can be obtained separately…

14 How to Condition CE100-SA Controlled & Zero EOF Capillaries For CE

Capillary Conditioning Procedure: Never Use Strong Bases on these Capillaries Carefully remove the capillary from the shipping/storage container. Remove protective polyimide coating by using 98% fuming…

15 How to perform an Ohm's Law plot for optimal CE results

Ohm’s Law Plot to Optimize your Separation MicroSolv CE Primer Select the optimal voltage setting for your separation Maximize the Efficiency of your method Learn the upper Voltage Limits of your method…

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