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Mobile Phase Handling

1 Can I use benzene as a mobile phase solvent?

Benzene can be used as an HPLC solvent in theory. It is compatible with standard HPLC instrumentation and will not damage an HPLC column. However, we would not recommend using this solvent for two main…

2 How should I handle the sparging stones/inlet filters to avoid contaminating my mobile phase?

The sparging stones/inlet filters should not be touched by human hands. Always wear clean standard laboratory gloves of use forceps when you handle them. Do not use powdered gloves, as these can introduce…

3 How To Condition 47mm Nylon Membranes for Use with the Mobile Phase Filter Degasser...

Filter membranes with smaller pores sizes such as 0.45µm have higher bubble points (the differential pressure required to draw air through a wet membrane). The bubble point is a function of the pore…

4 How to prepare 10 mM ammonium acetate in 90:10 acetonitrile : buffer

When you see something like "10 mM ammonium acetate in 90:10 acetonitrile : buffer" in a method, you may be wondering how exactly this mobile phase solution is prepared. For example, is it 10 mM with…

5 How to Use Bubble Point to Filter Water through Hydrophobic Membranes...

Bubble point pressure is the differential pressure required for a liquid to flow through a dry filter membrane. The differential pressure is either a positive pressure on the liquid layer on top of the…

6 Solvents and solutions that should be avoided when using PEEK fittings or tubing.

The solvents and chemicals that should be avoided when using PEEK tubing and fittings include: Nitric acid Sulfuric acid Halogenated acids, including hydrofluoric, hydrobromic, and hydroiodic acids Pure…

7 Use Teflon bottles for A and B solvents in negative ion mode LCMS

You will want to have both your A and B solvents (aqueous and organic) in Teflon mobile phase reservoir bottles. The reason for using these bottles is that sodium present in borosilicate glass mobile…

8 What is the useful temperature range of PEEK laboratory products?

The useful temperature range of PEEK related products for chromatography is up to 100 °C (tubing); up to 125 °C (fittings). PEEK is sometimes rated to 250°C but... It will not be as stable (lower burst…

9 Why is nylon called nylon?

According to the "Answers Corporation" website, nylon was developed at both New York and London, simultaneously. However, it has also been stated that the letters "nyl" were arbitrary and the "on" was…

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