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Cogent HPLC Column Recommendations

16 What are the main differences between HILIC and Aqueous Normal Phase?

There are many important differences between HILIC columns and Cogent TYPE-C Silica™ columns but for the sake of brevity, we will only address the main differences. The Cogent TYPE-C™ silica columns…

17 What can you recommend for a bromhexine HPLC assay method?

Question: I am currently using the following method for analysis of bromhexine. What column and/or alternative method conditions can you suggest? Mobile phase: Potassium di-hydrogen phosphate buffer(take…

18 What effect does high injection volume have when using the Cogent Diamond Hydride HPLC column in ANP?

When using the Cogent Diamond Hydride™ HPLC column in aqueous normal phase (ANP) mode, which is the best mode for hydrophilic analytes, you should expect the same sample loading capacity as you would…

19 What is the best way to separate phospholipids by HPLC?

Phospholipids present a challenge for chromatographers for various reasons. First, they have negligible UV absorption and generally require other detection methods. Second, subtle structural differences…

20 What is the most polar Cogent TYPE-C HPLC column?

The most polar HPLC stationary phase manufactured by MicroSolv Technology Corporation is the Cogent Amide™. It is very robust compared to most other "amide" columns and can be very effective for compounds…

21 When to use PEEK over stainless steel for HPLC column Harware.

Data in certain types of analyses may be adversely affected by use of stainless steel HPLC columns. In these cases, you may want to consider a column housed in PEEK hardware instead of traditional stainless…

22 Which column do you recommend for the separation of positional isomers by HPLC or LCMS?

The answer to this question depends on what type of positional isomers. For reversed phase separation of aromatic positional isomers, the Cogent Phenyl Hydride™ is often a good choice. These isomers…

23 Which HPLC column diameter should I use for LC-MS?

For most standard HPLC applications such as UV detection methods, a 4.6mm or 3.0mm I.D. is suitable. Lower diameters will present unnecessary problems for standard UV methods. For instance, the system…

24 Which HPLC column do you suggest for analysis of carotene or lycopene?

With these types of compounds, the main challenge will be separating the different isomers. There are numerous double bonds in the molecules, and many combinations of cis/trans isomers are likely to be…

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