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TYPE-C Silica Hydride HPLC Columns

16 I am seeing changing retention times and a peak showing up in my blank using the Cogent Silica-C column in normal phase. What can be the issue?

It is most likely a matrix effect. You probably should wash the column first with a reasonable content of the strong solvent of your mobile phase, higher than at the end of the gradient. Then you should…

17 I am using a Cogent TYPE-C™column with LC-MS and solvents containing 10 mM ammonium acetate. I noticed that my source was getting white film on it. At the same time the background noise was very low. Is my column bleeding the packing material?

Cogent TYPE-C™ columns including the Cogent Diamond Hydride have a very low background noise even when used with LC-MS and the white film most likely comes from ammonium acetate build up from your mobile…

18 Is TYPE-C Silica™ high purity and low metal content silica like Type silica?

Yes, it is and it has all the advantages of ordinary, market leading high purity, type-B silica supports for HPLC. © Copyright MicroSolv Technology Corporation, all rights reserved.

19 Many other column suppliers suggest different columns for Acids, Neutrals or Bases, which TYPE-C™ column is best for each of these compound groups?

The Cogent TYPE-C™ HPLC phases are unique in that they have different retention mechanisms which can be exploited to give unique separations. It may not be necessary to use different columns for different…

20 Policy on verifying a new Cogent HPLC column's performance

When you first receive a Cogent™ HPLC column, its performance should be checked by running a quick test to measure peak symmetry and efficiency. The column you purchased came with a Packing Test Chromatogram…

21 Since you manufacture the Cogent Type-C™ silica using a transition metal catalyst, what if any is the presence of any trace quantity on the surface and how did you measure this?

This is an issue we confronted a long time ago. We were also concerned about this potential problem. First we intentionally ran the bonding reaction at high catalyst concentration in order to actually…

22 What are inosine nucleotides?

All nucleotides are are comprised of the following sub-structures: A nucleobase, a 5-membered ring sugar unit (either ribose or 2-deoxyribose), and one or more phosphate groups. In the case of inosine…

23 What are some examples of third-party journal articles that feature the Cogent™ columns in metabolomics studies?

There are many examples of the Cogent™ columns used in metabolomics research, which have been published by third-party researchers in peer-reviewed scientific journals. Two notable works are described…

24 What are the cost advantages of Cogent TYPE-C Silica columns over HILIC, mixed mode, and ion exchange for LC-MS and preparative applications?

There are several reasons why the Cogent TYPE-C™ Silica is superior to HILIC, Mixed Mode, or Ion Exchange phases in terms of total cost to the end user. 1. With HILIC columns, you need to have 25–50…

25 What are the main causes of column blockage and how can I minimize them?

A column blockage can be a problematic issue, resulting in undesirable phenomena such as high backpressure. The most important thing you can do is to prevent the problem from occurring in the first place.…

26 What is a "direct silicon-carbon bond" regarding Cogent HPLC columns?

For chemical bonds in traditional, ordinary type B silica based materials, the organosilane starting material is Si-O-Si-C and this reagent is reacted with the silica to form the final stationary phase…

27 What is ANP or Aqueous Normal Phase?

Aqueous normal phase chromatography (ANP) is a chromatographic technique which encompasses the mobile phase region between reversed-phase chromatography (RP) and organic normal phase chromatography (ONP)…

28 What is Aqueous Normal Phase (ANP) and how can it help you?

Aqueous normal phase chromatography From Wikipedia, the free encyclopedia Aqueous normal phase chromatography (ANP) is an HPLC technique which encompasses the mobile phase region between reversed-phase…

29 What is the appropriate stop depth/seating depth for your HPLC column end fittings?

The stop depth or seating depth is the distance between the end of a ferrule and the end of a section of tubing in the inlet port of the column. If the stop depth is too short, there will be a gap between…

30 What is the biggest difference between ordinary HPLC silica columns and Silica-C™?

The biggest difference is the surface chemistry and how it will benefit you. The dominance of silicon hydride (Si-H) groups instead of the “silanol group” (Si-OH) common to all irregular or spherical…

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