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Controlled Flow Capillaries

1 Are the Zero and Controlled Flow Capillaries compatible with organic solvents?

The sulfonic acid polymer and/or linear polyacrylamide polymer is covalently bonded to the capillary wall and therefore is fully compatible with organic solvents such as acetonitrile and DMF. Essentially,…

2 At what temperature do I store the neutral-coated Controlled Flow Capillaries?

The capillaries should be stored at room temperature (approximately 22C) in a dry place. It does not depend on the ID of the capillary. Many customers actually tape them to the wall near their CE instrument.…

3 At which pH will my method optimize with these capillaries?

These capillaries columns are pH independent. What that means is the flow does not change significantly with different pH levels. The optimal pH will be the pH at which your analytes separate best. Click…

4 Can I use a laser to make a window for the Controlled Flow/Zero Flow Capillaries?

No, these capillaries have a special covalently bonded coating on the inside capillary wall (linear polyacrylamide) that may be damaged using a laser. The outer polyimide coating should be removed only…

5 Can I use the MicroSolvCE Window Maker or similar units on the Zero or Controlled Flow Capillaries?

It is not normally recommended that one use high heat to remove the polyimide coating on the capillary. This could create a “void” in the wall coating and produce erroneous results. It is best to…

6 Do you sell CE capillaries with pre-made windows?

We do not sell CE capillaries with a pre-made window for a few reasons; most importantly, the window is fragile often causing damage during shipping. In order to ensure quality capillaries are delivered…

7 For the bonded or coated capillaries, do you have other lengths for sale?

The bonded or coated capillaries are only available in 1M lengths. The reason is that it is very difficult to push the bonded reagent through a 50um or 75um tube and get proper coating. Ordering Information…

8 How can I remove polyimide from your capillaries without using heat?

To remove polyimide coating from our Simplus™ brand or Zero™ or Controlled Flow™ capillaries for CE without using heat, it is best to use fuming sulfuric acid at 100°C. You may want to heat the…

9 How do I remove a blockage in my CE capillary?

If you observe a sudden drop in current, the problem could be due to a particle causing a blockage in the capillary. If this occurs, try flushing the capillary with filtered, CE-grade DI water. To do…

10 How do the capillaries provide different amounts of flow?

We coat the bare fused silica with a proprietary polymer that has a large amount of Sulfonic acid groups exposed to the lumen of the capillary. The different flow rates of the columns are due to differing…

11 How does the Controlled Flow CE Capillary work?

The Controlled Flow™ CE capillary is made from a standard bare fused silica CE capillary but has an inner coating which is covalently bonded with a neutral polymer that has a controlled amount of sulfonic…

12 Is the conditioning of the Controlled and Zero capillaries the same as with a bare fused silica capillary?

No. Inside the front cover of the column case is a special set of conditioning steps that must be taken. It is important that these conditions be followed. You can also see these conditions on our website…

13 What are the terms such as high flow rate in Controlled Flow capillaries based on?

The terms High, Medium and Low are based on a relative flow when compared to Bare Fused Silica Capillaries. The Controlled Flow capillary always is slower than bare silica but the High Rate is the highest…

14 What is a Zero EOF capillary mostly used for?

There are many uses for Zero EOF in HPCE. One use is to allow analytes to separate based on electrophoretic mobility only. The Zero EOF capillary is an excellent choice for this technique because, regardless…

15 When should I use CElixir™ dynamic coatings vs. Controlled EOF™ capillaries in my CE method?

Controlled EOF™ capillaries are used when you want to suppress the EOF partially or completely and you want to use IEF (iso-electro focusing). Since separation takes place strictly due to electrophoretic…

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