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CElixir SDS

1 Can CElixir-SDS™ be used for Proteins?

Yes, several articles describe using MEKC with SDS for protein separations. Our R&D lab will document this further. Click here for more information about CElixir-SDS © Copyright MicroSolv Technology…

2 Can I add chiral selectors such as cyclodextrin to the run buffer for chiral analysis?

Yes, you can add reagent grade or better CD (cyclodextrins) listed below for chiral and enhanced separations. Beta-Cyclodextrin Gamma-Cyclodextrin dimethyl Beta-Cyclodextrin hydroxypropyl Beta-cyclodextrin…

3 Can I add SDS to change the molarity?

Yes, you can add reagent grade SDS and change the SDS concentrations. This may change the overall EOF and the run times and other expected results. Click here for more information about CElixir-SDS ©…

4 Can I get a different formulation of CElixir-SDS™ for my specific application?

Yes, our R&D lab is very happy to work with you and develop a “custom” kit for your application needs. For more information, contact our Technical Service Department . Click here for more information…

5 Can I return the capillary to its original state after I use CElixir™ products?

Yes, you can if you follow the procedure in the CElixir products Operating Manuals. However, it is recommended that once you use CElixir™ on a capillary, you dedicate that capillary for the exclusive…

6 Can temperature have an effect on CElixir-SDS™?

Yes, as with any CE method, temperature is something that should be controlled during each test run. Click here for ordering information for CElixir Dynamic Coatings © Copyright MicroSolv Technology…

7 How can I analyze iron (FeII) and iron (FeIII) by CE?

There are two viable options for analysis of iron (II) and iron (III) by CE. Option 1. Add EDTA and analyze the iron-EDTA complexes. Use the CElixir OA Kit because the complexes will be anionic. Option…

8 How does CElixir-SDS™ separate neutral molecules using CE?

The CElixir-SDS™ kits contain SDS (sodium dodecyl sulfate) at concentrations that are at or above the critical micelle concentration. Micelles form with hydrophobic centers and charged groups on the…

9 How many coatings should I expect from 1.4ml of CElixir-SDS Initiator™ solution? How long will a vial of this solution last?

If you follow our Operating and Instruction Manual for CElixir-SDS™, the coating solution (Initiator (A)) is used only once (1) per day or when a new capillary is used. Once you coat your capillary…

10 How many test runs should I expect from one CElixir-SDS™ kit?

CElixir-SDS™ is designed to provide between 150 and 250 test runs. The actual outcome is determined by the waste in your particular system, how careful you can be, the length of your capillary, run…

11 I want to use CElixir-SDS™ but I need to work at pH 6.5. How can I do this?

You can lower the pH of the Accelerator buffer with phosphate however it is difficult to measure pH due to the SDS. Therefore we suggest that you contact our technical department or use this link to inquire…

12 Is there anything in CElixir-SDS™ that can pit or damage a bare fused silica capillary in any way?

No, CElixir-SDS™ is designed to work with bare fused silica capillaries and is not recommended for permanently coated capillaries. Click here for more information about CElixir-SDS © Copyright MicroSolv…

13 Separation of Neutral Compounds by Capillary Electrophoresis using CElixir-SDS™

Neutral compounds normally migrate with the Electro-Osmotic Flow (EOF) in CZE. Using the proper buffer additives, MEKC can employed and neutral compounds can be separated along with charged molecules.…

14 What are the storage conditions for CElixir™ brand products?

CElixir™ can be stored at room temperature along with other reagents. If you are going to leave the bottle of CElixir™ open for an extended period, you may want to add a little sodium Azide to minimize…

15 What can I dilute the run buffer with? Can I adjust the pH?

One of the advantages of CElixir-SDS™ is that you can adjust the kit for your specific separation needs. You can use any of the solutions below to adjust the method: Methanol (up to 20%) Acetonitrile…

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