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CElixir plus

1 Can I return the capillary to its original state after I use CElixir™ products?

Yes, you can if you follow the procedure in the CElixir products Operating Manuals. However, it is recommended that once you use CElixir™ on a capillary, you dedicate that capillary for the exclusive…

2 How can I analyze iron (FeII) and iron (FeIII) by CE?

There are two viable options for analysis of iron (II) and iron (III) by CE. Option 1. Add EDTA and analyze the iron-EDTA complexes. Use the CElixir OA Kit because the complexes will be anionic. Option…

3 I am using CElixir-pH™ and I want to use CElixir-plus™ on the same capillary. Can I do this?

Yes, but it is recommended to use a separate capillary for optimal performance, however you could use following conditioning of the capillary. Rinse the capillary for 1 minute with 0.1N NaOH. Wait for…

4 Indirect detection will produce “negative peaks”. Is it possible to view and print these as “normal peaks”?

Yes, using the software of most modern CE instruments, find the setting for “Indirect Detection” and the peaks appear as if they were absorbing UV in normal mode. View more information about CElixir-plus…

5 Is it recommended to use a coated capillary with CElixir-plus?

No, CElixir-plus™ is designed to produce maximum results with a good quality, CE grade, bare fused silica capillary such as the Simplus brand. Click here to read about Simplus brand capillaries View…

6 We have had some trouble getting our sample into solution with CElixir-pH™. What do you suggest?

One of the greatest advantages of CElixir™ is that you can add organic solvents to assist you with solvating your sample and you will not sacrifice EOF. Ethylene Glycol, Methanol and Acetonitrile have…

7 What are the storage conditions for CElixir™ brand products?

CElixir™ can be stored at room temperature along with other reagents. If you are going to leave the bottle of CElixir™ open for an extended period, you may want to add a little sodium Azide to minimize…

8 What is CElixir-plus™ for capillary electrophoresis?

CElixir-plus™ is a kit of pre-made buffers that are used to separate positively charged molecules and aliphatic amines. The kit uses a dynamic coating in order to stabilize the EOF. There are no need…

9 What is the buffer concentration of CElixir-plus™?

CElixir-plus™ Initiator and Accelerator are buffered in 4-aminopyridine, the separation buffer is 20mM Malic Acid, 4-aminopyridine, 19-crown ether. View more information about CElixir-plus © Copyright…

10 What is the pH range of CElixir-plus™?

The coating solutions supplied with CElixir-plus™ are at pH 4.2. The supplied separating buffer is 20mM malic acid pH 4.3. The Conditioner solution is LiOH, 0.1mol/L. Download the Operating Manual for…

11 What is the purpose of the CElixir-plus™ conditioning solution?

The CElixir-plus™ conditioning solution, contains LiOH and is used between separations to clean the capillary which facilitates the dynamic coating process and helps to eliminate carry over of Sodium.…

12 What is the UV wavelength that I should use for CElixir-plus™?

The chromophore in CElixir-plus is maximized at 200nm. View more information about CElixir-plus © Copyright MicroSolv Technology Corporation, all rights reserved.

13 When I am running CElixir-plus™, should I use a post injection water plug?

Yes, not only will the stacking help the run but the post injection water plug always helps injection reproducibility. View more information about CElixir-plus © Copyright MicroSolv Technology Corporation,…

14 When should I use CElixir™ dynamic coatings vs. Controlled EOF™ capillaries in my CE method?

Controlled EOF™ capillaries are used when you want to suppress the EOF partially or completely and you want to use IEF (iso-electro focusing). Since separation takes place strictly due to electrophoretic…

15 Which reagents are required when using CElixir™ products?

No, All of the reagents that are needed to achieve great CZE with CElixir™ are contained in the CElixir HPCE Column Kit: The Initiator Solution, The Accelerator Solution and CE grade water. © Copyright…

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