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CElixirOA

1 Can I buy CElixir OA™ Accelerator or Initiator solutions separately?

The pricing of the CElixir OA™ Kit is based on the amount of Accelerator. The Initiator may be in excess in order to ensure the user has enough of both reagents. For this reason, you may find that when…

2 Can I return the capillary to its original state after I use CElixir™ products?

Yes, you can if you follow the procedure in the CElixir products Operating Manuals. However, it is recommended that once you use CElixir™ on a capillary, you dedicate that capillary for the exclusive…

3 Can I use 254nm as my wavelength with CElixirOA™?

Yes, but the maximum for sensitivity is 233nm for most molecules. However, we have seen 254nm to be best for nitrate analysis. Click here to view more information about CElixirOA © Copyright MicroSolv…

4 Can I use CElixirOA™ for the analysis of Bromide?

Yes, our customers have been successful with Bromide using CElixirOA 5.4 product. However, please note that it may co-migrate with chloride. However, to increase resolution, you can use a longer capillary…

5 Can I use CElixirOA™ for the analysis of Cyanide?

We have not been successful with Cyanide using any of the CElixirOA products. Using pH 12 buffers may be called for but we do not offer such a product at this time. Click here to view more information…

6 Can I use CElixirOA™ for the analysis of Flouride?

Yes, our customers have been successful with Flouride using CElixirOA 5.4 product. However, please note that it may co-migrate with formiate. To increase chances of separation, a longer capillary and…

7 Can I use the Anions Test Mixture 8 with the CElixir OA pH 5.4 buffer?

We recommend the Anions Test Mixture 5 for use with the pH 5.4 CElixirOA™ kit. However, you can use the Anions Test Mixture 8 with the kit as well. Just be aware that the migration times will be different…

8 Can I use the CElixirOA Kit pH 5.4™ kit be used for quantitation of a sample with 1 mM sulfate and 150 mM Acetate?

It may be challenging to obtain that level of quantification. Try using electrokinetic injection on a sample (10–50X dilution) with an internal standard of fluoride or malic acid. The acetate ion will…

9 Can temperature have an effect on CElixirOA™?

Yes, CElixirOA™ 5.4 and 8.2 are made with a Tris buffer. Since a 10°C change in temperature can effect a 0.3pH unit drop, this may effect your separation. You can bring the pH higher by adding solid…

10 How can I analyze iron (FeII) and iron (FeIII) by CE?

There are two viable options for analysis of iron (II) and iron (III) by CE. Option 1. Add EDTA and analyze the iron-EDTA complexes. Use the CElixir OA Kit because the complexes will be anionic. Option…

11 How can I separate sulfates, phosphates, and fluorides by CE?

Analysis of sulfates, phosphates, and fluorides is best done with CElixirOA™ 5.4 and 8.2, but not CElixirOA™ 2.5. For phosphates and fluorides, the acidic rinse with 0.1M HCl is mandatory for good…

12 How can I use the CElixirOA™ kit for both amines and organic acids?

You can use the kit with amines and organic acids. Please note that CElixirOA™ kit is designed for use in reverse EOF and polarity. That means that negatively charged species (anions) will migrate to…

13 How do you calculate the limit of detection for a CE method using CElixirOA™?

I usually accomplish this with a calibration with 5 levels. Then I do a repeatability test on the level that will give an area close to 500 and calculate the mean, the standard deviation and the CV. I…

14 I am analyzing wine, will the limit of detection (LOD) when I use CElixirOA™ allow for the proper analysis of common organic acids and anions in wine ?

Yes, we usually work in ppm using corrected area with CElixirOA™. For calibration one should expect a R2 of 0.999 or more between 5 and 200ppm. Depending on injection of sample, LOD may be 1ppm and…

15 I am using a very large sample plug (20 psi seconds) which is diluted 1:10 and 1:20 in water. I notice that in the beginning of the run the current is rather low. The current gradually increases then stabilizes. Is this normal?

No, what may be happening is that since the sample plug is extraordinarily large and the sample is further diluted with water, it will take time for sample plug to become totally ionized. During this…

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