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Cogent Bidentate C18

1 How do I condition new Cogent TYPE-C™ Columns for use?

For all new Cogent TYPE-C™ HPLC columns with the exception of new Diamond Hydride™ columns, all one needs to do is run 7-10 column bed volumes of your mobile phase through the column at your normal…

2 My method uses an ordinary type-B silica based HPLC column. Is there a Cogent TYPE-C™ column equivalent substitute?

While many columns use the same nomenclature for their stationary phases such as C18, phenyl, C8 etc, every brand of column has unique properties and in any given method, columns can perform equally and…

3 What is the biggest difference between current HPLC phases and Bidenatate C18™?

The biggest difference is the silica surface and how it will benefit the user. The main difference in the silica’s is the dominance of Silicon-Hydride (Si-H) groups on the TYPE-C™ products instead…

4 Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column?

The Cogent Bidentate C18™ phase can be stored in un-buffered aqueous / organic solvents, but flowing significant bed volumes of un-buffered aqueous will cause a drop in efficiency of approximately 35%…

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