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CElixir-pH

1 Adsorption equilibria in CE using CElixir-pH.

The answer is very simple, the adsorption equilibria is shifted from your analyte to The Accelerator Solutions. The reasons for this are many but the main reason is the number of charges on your sample…

2 Can CElixir-pH™ be used with SDS?

No, it cannot be used with SDS sodium dodecyl sulfate. If your current method uses SDS but you are not happy with the results of that method, you can choose from other chiral selectors that are compatible.…

3 Can I return the capillary to its original state after I use CElixir™ products?

Yes, you can if you follow the procedure in the CElixir products Operating Manuals. However, it is recommended that once you use CElixir™ on a capillary, you dedicate that capillary for the exclusive…

4 Can I use organic solvents with CElixir-pH™?

Yes, you can use small amounts of solvents for solubility and efficiency additives. Be careful not to add so much solvent that you dilute CElixir™ © Copyright MicroSolv Technology Corporation, all…

5 Do I use a buffer with CElixir-pH in Capillary Electrophoresis™?

No, there is no need for other buffers while using the CElixir™ HPCE Column Kit. The Accelerator Solution has the “run buffer” built into it as a part of its formula. © Copyright MicroSolv Technology…

6 How do I eliminate the band broadening I get on subsequent runs when working with high concentrations of proteins using CElixir-pH?

Use a Water Rinse. It is recommended that when your samples are high concentrations of protein or simply a very “sticky” protein, that immediately before you inject the sample, between runs, that…

7 How do I get started with CElixir-pH™?

It is very easy to use and get started with CElixir™. Simply start with The Initiator Solution (A) and The Accelerator Solution (B) with the pH that is closet to the pK of your analyte. If you do not…

8 How to get a pH other than that of the pH on the bottle of The Accelerator Solution (B) in CElixir-pH.

You can easily get a different pH of The Accelerator Solution (B) by mixing other Accelerator Solutions of higher or lower pH or by adding H3PO4, HCL or NaOH. The CElixir-pH Operating and Troubleshooting…

9 In your Operating manual for CElixir-pH™, you suggest a water plug. What is the purpose of this step?

The water plug is to avoid “sample carry over” into the anodic buffer. This water plug also helps to improve resolution, but mostly a water plug improves injection reproducibility. Click here to download…

10 Is CElixir-pH™ compatible with Urea?

Yes, also with Taurine, Betaine and Ethylene Glycol Guanidine up to 10%. This is only a partial list. © Copyright MicroSolv Technology Corporation, all rights reserved.

11 Is there any way I can minimize my serum protein samples from aggregating in CElixir-pH™?

Yes, when separating serum proteins or iso amylase isoCk or iso alkaline phosphotase, it may happen that a protein (immunoglobulin or enzyme) is bound to another (such as an immune complex) or is present…

12 My method calls for a citrate buffer. Will CElixir-pH™ give the exact same migration time and peak area results if I run it at similar pH?

No. CElixir™ creates an entirely different CZE environment and will be very different. Only the end results should be compared to your original method. A new method will have to be used for CElixir™.…

13 What are the storage conditions for CElixir™ brand products?

CElixir™ can be stored at room temperature along with other reagents. If you are going to leave the bottle of CElixir™ open for an extended period, you may want to add a little sodium Azide to minimize…

14 What current should I expect, using CElixir-pH™, at 25kV in a 50um ID Capillary, 27cm long?

The current you should expect at every pH is approximately 80-90uAmps. © Copyright MicroSolv Technology Corporation, all rights reserved.

15 What effect does CElixir-pH™ have on positively charged analytes like proteins?

Proteins that are positively charged are ion paired with the Accelerator Solution (B). © Copyright MicroSolv Technology Corporation, all rights reserved.

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