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HPLC Tips and Tricks

16 What is the most effective way to clean HPLC columns after or before use?

Many commonly used cleaning methods feature a strong wash solvent, run isocratically in order to elute strongly retained compounds or residual solvents from the column. After some time of washing in this…

17 Will phosphate precipitate in an HPLC column?

Phosphoric Acid will not precipitate in the columns. Sodium phosphate (which is not recommended) but which is often used as a buffer, will precipitate in the columns and should be avoided as a storage…

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