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MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

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Applications for RP Reversed Phase HPLC

1 (l)-9-Carboxy-11-Nor-Delta-9- tetrahydrocannabinol

THC metabolite in urine matrix Discussion The extraction procedure using an SPE technique allows for successful detection of this cannabis metabolite with the Cogent Phenyl Hydride™ column. It is worth…

2 β-Carotene Capsule

Separation from other matrix peaks Discussion Beta-carotene may be taken as a dietary supplement in capsule form. In this case, a wide variety of matrix peaks were observed in the chromatographic data.…

3 1,4-Benzodiazepines in Urine

LC-MS method with SPE Discussion The Cogent Diol™ column was used in analysis of 1,4-benzodiazepines in urine samples after SPE extraction using two types of retention modes. Four available compounds…

4 10 Phenolic Compounds Separated

Commercial Rice Extract Discussion Commercial rice extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride™ column was an excellent choice to use for analysis of phenolic compounds…

5 4’-Aminoacetophenone: Drug Metabolite

4’-aminoacetophenone Discussion The use of a Cogent Bidentate™ C18 column in RP-HPLC mode was investigated using 4’-acetaminophenone as a model compound. The short column gave adequate retention…

6 5 Phenolic Compounds Separated

Commercial Rice Extract Discussion A commercial rice extract was analyzed using the Cogent Phenyl Hydride™ column and peaks were assigned based on retention times and m/z values for the compounds of…

7 A Generic Method Development Strategy: Aqueous Reverse Phase (ARP), Aqueous Normal Phase (ANP) & Organic Normal Phase (ONP)

Choose Polarity or Functionality as the Main Selectivity Factor on the Same Column Discussion Chromatogram A ARP- Reverse Phase: Loratadine in 100% THF elutes with the solvent front. With 70% Aqueous/…

8 Acetaminophen & impurity

Drug Substance by HPLC Discussion A high-performance liquid chromatography (RP-HPLC) method has been developed for determination of acetaminophen: drug substance. The chromatographic separation was achieved…

9 Acetaminophen Impurities Method

Robust, Easy, APAP Method Discussion Acetominophen and two of its major impurities were analyzed using the Cogent Bidentate C18™ column and a simple mobile phase. The peak shapes were very high. The…

10 Advantage of Using Cogent HPLC Columns for Drugs of Abuse Analysis

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze…

11 Advantages of using Cogent HPLC Column to Analyze Amazonian Bark Extract

The Amazon rainforest is the most biodiverse region in the world. Over one quarter of natural medicines have been found in rainforests, and new species are constantly being discovered. Therefore, there…

12 Advantages of using Cogent HPLC column to Analyze Pharmaceuticals in Plasma

One of the most important applications of LC-MS today is the quantitative analysis of plasma samples. Pharmaceuticals and their metabolites may need to be monitored in plasma in fields such as clinical…

13 Aegeline in Protein Sport Mix

LC-MS analysis of harmful substance in food supplement Discussion The data demonstrates the use of the Cogent Bidentate C18 2.o™ column for analysis of dietary supplement samples. Isolation of the aegeline…

14 Amoxicillin (USP Method)

Robust USP Assay Analysis Discussion The USP assay method for amoxicillin often yields poor peak shapes and low method reproducibility with L1 columns based on type B silica. In contrast, the figures…

15 Analgesic Drugs

Excellent Peak Shapes Discussion A robust and rapid method was developed for the accurate simultaneous determination of 2 analgesics (Paracetamol, Aspirin), caffeine, internal standard (benzoic acid),…

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