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Applications for ANP Aqueous Normal Phase

1 1,4-Benzodiazepines in Urine Analyzed by LC-MS using SPE.

Tamemazepam, Diazepam, Nordiazepam & Midazolam Peak: 1 . T e m a z e p a m 3 0 1 . 0 7 3 9 m / z [ M + H ] + 2 . D i a z e p a m 2 8 5 . 0 7 9 0 [ M + H ] + 3 . N o r di a z e p a m 2 7 1 . 0 6 3…

2 1,4-Benzodiazepines in Urine Analyzed by LCMS.

LC-MS method with SPE 1 , 4 - B e n z o d i a z e pi n e s i n U r i n e Peak: 1 . T e m a z e p a m 3 0 1 . 0 7 3 9 m / z [ M + H ] + 2 . D i a z e p a m 2 8 5 . 0 7 9 0 [ M + H ] + 3 . N o r di a z…

3 3-Hydroxy-3-Methylglutaric acid (HMG) in Urine

Important biomarker for diabetes and HMG disorder © Copyright MicroSolv Technology Corporation, all rights reserved.

4 3,3'-Diaminobenzidine (DAB) - LC-MS method with excellent retention and peak shape

Peak: 3,3'-Diaminobenzidine 215.1291 m/z (M+H)+ t0: 0.9 min Method Conditions Column: Cogent Diamond Hydride™, 4µm, 100Å Catalog No.: 70000-15P-2 Dimensions: 2.1 x 150 mm Solvents: A: 50% DI H2O/…

5 A Generic Method Development Strategy: Aqueous Reverse Phase (ARP), Aqueous Normal Phase (ANP) & Organic Normal Phase (ONP)

Choose Polarity or Functionality as the Main Selectivity Factor on the Same Column © Copyright MicroSolv Technology Corporation, all rights reserved.

6 Acetone as the Organic Mobile Phase Component

“Green” substitute for separation of amino acids Caution: Acetone and Amines can react When working with Acetone as an HPLC solvent it is important to note that primary amines could react with Acetone…

7 Acetyl-L-Carnitine (ALC) in Plasma

Excellent LC-MS method in spiked plasma sample © Copyright MicroSolv Technology Corporation, all rights reserved. For more detailed application, click here.

8 Acrylamide HPLC analysis method and application note

Easy & Precise Retention © Copyright MicroSolv Technology Corporation, all rights reserved.

9 Advantage of Using Cogent HPLC Columns for Drugs of Abuse Analysis

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze…

10 Advantages of using Cogent HPLC Column to Analyze Amazonian Bark Extract

The Amazon rainforest is the most biodiverse region in the world. Over one quarter of natural medicines have been found in rainforests, and new species are constantly being discovered. Therefore, there…

11 Alprazolam (Xanax®)

Robust Separation of API from USP Internal Standard © Copyright MicroSolv Technology Corporation, all rights reserved.

12 Alprazolam (Xanax®) HPLC Method Transfered.

Use of 2.2um high efficiency column. © Copyright MicroSolv Technology Corporation, all rights reserved.

13 Amikacin & Tobramycin

Highly polar antibiotic separation © Copyright MicroSolv Technology Corporation, all rights reserved.

14 Amiloride and Triamterene

LC-MS method for spiked urine sample © Copyright MicroSolv Technology Corporation, all rights reserved.

15 Amino Acids Non Derivatized

From Synthetic Urine © Copyright MicroSolv Technology Corporation, all rights reserved.

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